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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/10/2020 |
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Data da última atualização: |
19/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Insect Science, v. 27, n. 3, p. 519-530, 2020. |
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DOI: |
https://doi.org/10.1111/1744-7917.12654 |
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Idioma: |
Inglês |
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Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02437naa a2200289 a 4500
001 2125614
005 2020-10-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/1744-7917.12654$2DOI
100 1 $aDORTA, S. de O.
245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource]
260 $c2020
520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.
650 $aCitrus
650 $aDiaphorina citri
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aBALBINOTTE, J.
700 1 $aMONNERAT, R.
700 1 $aLOPES, J. R. S.
700 1 $aCUNHA, T. da
700 1 $aZANARDI, O. Z.
700 1 $aMIRANDA, M. P. de
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tInsect Science$gv. 27, n. 3, p. 519-530, 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 18 | |
| 1. |  | ÁVILA, Z. R. de; BRAÚNA, L. M.; ORIOLI, F. P.; FALCAO, J. S. V.; MELLO, S. C. M. Antagonismo de Trichoderma spp a Sclerotium rolfsii in vitro. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S175, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | ÁVILA, Z. R. de; FALCÃO, J. V.; BRAÚNA, L. M.; SILVA, M. C. F.; MELLO, S. C. M. Antagonismo de Trichoderma spp a Sclerotinia scierotiorum, in vitro. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S174, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | AVILA, Z. R. de; MELLO, S. C. M. de; RIBEIRO, Z. M. de A.; FONTES, E. M. G. Produção de inóculo de Alternaria cassiae. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 3, p. 533-541, mar. 2000 Título em inglês: Production of Alternaria cassiae inoculum.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | ÁVILA, Z. R. de; PITELLI, R. A. Crescimento, esporulação e virulência do inóculo de Cercospora piaropi, agente de biocontrole do aguapé. Fitopatologia Brasileira, Brasília, v.29, n.2, p. 189-192, mar./abr., 2004.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | CARVALHO, D. D. C.; INGLIS, P. W.; ÁVILA, Z. R. de; MARTINS, I.; MUNIZ, P. H. P. C.; MELLO, S. C. M. de. Morphological characteristics and genetic variability of Trichoderma spp. from conventional cotton crop soils in Federal District, Brazil. Journal of Agricultural Science, v. 10, n. 8, p. 146-155, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | FALCÃO, J. V.; ÁVILA, Z. R. de; ORILI, F. P.; MELLO, S. C. M. de. Métodos de contaminação do solo para avaliação da patogenicidade de Sclerotinia Sclerotiorum e Sclerotium rolfsii à soja. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S174, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | MARTINS, I.; PEIXOTO, J. R.; ÁVILA, Z. R. de; MELLO, S. C. M. de; PÁDUA, R. R. de. Esporulação de Colletotrichum gloeosporioides em meios líquidos. Summa Phytopathologica, Botucatu, v. 33, n. 2, p. 203, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 18 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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